Genotyping and primer details


Genotyping and primer details for SALK_013217c - At3g25750
Line ID SALK_013217c
Locus At3g25750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9401233 of line SALK_013217c Chr3:9401233
   
Insertion allele amplicon LBb3 - 49jd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 49jd: 5'-TCTTCAAACATCCATGGTACTCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9401942
   
wt amplicon (over InsPos) 11km - 49jd
wt amplicon start Chr3:9400804
wt amplicon end Chr3:9401942
wt amplicon size 1139 bp
Locus specific primer 49jd: 5'-TCTTCAAACATCCATGGTACTCAG-3'
2nd locus specific primer 11km: 5'-AATTATGCCACAATCCACATGTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11