Genotyping and primer details


Genotyping and primer details for SALK_013442 - At1g08130
Line ID SALK_013442
Locus At1g08130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2543794 of line SALK_013442 Chr1:2543794
   
Insertion allele amplicon LBb3 - 13hd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 13hd: 5'-CTTGGTACAAATCAGCAATCTGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2542963
   
wt amplicon (over InsPos) 01ix - 13hd
wt amplicon start Chr1:2542963
wt amplicon end Chr1:2544141
wt amplicon size 1179 bp
Locus specific primer 13hd: 5'-CTTGGTACAAATCAGCAATCTGAA-3'
2nd locus specific primer 01ix: 5'-AAACATTGGACTCAGATGCTACCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11