Sequences from confirmation amplicon


Confirmation sequences for line SALK_013442 and hit At1g08130 are shown.
Line ID SALK_013442
Locus At1g08130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2543794
Primers used for PCR 13hd - LBb3, show primer details
   
Sequence Name 88-K112710-SALK_013442C-13hd
GenBank Accession LN486318 [GenBank]
Sequencing primer gene specific primer, 13hd: 5'-CTTGGTACAAATCAGCAATCTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K112710-SALK_013442C-13hd
GAGTTTCGAACCAGTCTGGTCTTGTCTTTCAGTATTGAAAACATATGAGTGTAATTAGAA
TGAAACATCACCTGCTCAGATGATGTTGCTTCCTCGGGCTTCTTGTCTTCCCGTACACGA
AGTAGACGAGGAAAACGAAGAGAAATCCCCTTTTAACGAAACAAAATTGAAAACCAATAC
GTAACAATATATCAGCAGAGATATTCAAACCAAAAAGGAAAAAGAAGAAAATAAGTCGGA
GAAAGTTAAACCTTATCAGGATCCACTATGCCAGTAGCTGCACGGTGCACAGGGCTAATT
GTTAAGTCAGCTGCTTTCACTTCCCAGACCTTTAAGCAAACATATTTTTTACCTTTCATT
TCAACCCATACGATAGAATGGCGAAACTAATAAATATAAGATAACATTTTGAGTACAAGC
AAACATAATCCTACCTCAGTGGGCTCAAACCAAACATCTGGATTGAGGCTGTCTCCAACT
CGGTAGTATTGCTGGATATTTGAATCATTACAATTAAATAAGAATTCATGCTAGAGACAT
ATATGATAAGGAAGAAGAAAATATCCTACTTTTGGAGTAGCAATCACTTGAGAGCGAAGA
CTGGACGACCGCTCGTCAAGCATGGCATCAGAAAATCCAGTACCTAGTAGCCCAAACCGT
ATAACCAAGGTTTAAGAAACTTGAAAAACTGAACACACATACATAATATGAAATACCAAG
GTGTATTCATGTATTCGACTGACCAATTTTACAAATGCTCTGGAACTCTTCCTTATCAAC
ATCATAGCAAGCGGACACCTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGT
TTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGA
GAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGT
GGTT
   
Sequence Name 78-K112709-SALK_013442C-R210
GenBank Accession LN486189 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K112709-SALK_013442C-R210
ATAGGAGAGTTCCAGAGCATTTGTAAATTGGTCAGTCGAATACATGAATACACCTTGGTA
TTTCATATTATGTATGTGTGTTCAGTTTTTCAAGTTTCTTAAACCTTGGTTATACGGTTT
GGGCTACTAGGTACTGGATTTTCTGATGCCATGCTTGACGAGCGGTCGTCCAGTCTTCGC
TCTCAAGTGATTGCTACTCCAAAAGTAGGATATTTTCTTCTTCCTTATCATATATGTCTC
TAGCATGAATTCTTATTTAATTGTAATGATTCAAATATCCAGCAATACTACCGAGTTGGA
GACAGCCTCAATCCAGATGTTTGGTTTGAGCCCACTGAGGTAGGATTATGTTTGCTTGTA
CTCAAAATGTTATCTTATATTTATTAGTTTCGCCATTCTATCGTATGGGTTGAAATGAAA
GGTAAAAAATATGTTTGCTTAAAGGTCTGGGAAGTGAAAGCAGCTGACTTAACAATTAGC
CCTGTGCACCGTGCAGCTACTGGCATAGTGGATCCTGATAAGGTTTAACTTTCTCCGACT
TATTTTCTTCTTTTTCCTTTTTGGTTTGAATATCTCTGCTGATATATTGTTACGTATTGG
TTTTCAATTTTGTTTCGTTAAAAGGGGATTTCTCTTCGTTTTCCTCGTCTACTTCGTGTA
CGGGAAGACAAGAAGCCCGAGGAAGCAACATCATCTGAGCAGGTGAAGTTTCATTCTAAT
TACACTCATATGTTTTCAATACTGGAAAGACAAGACCAGACTGGTTTTGAAACTCTTTTC
CTTTTTCTAATTCTCAATTTTCCCTGCACATTTTCAGATTGCTGATTTGGTACCA

Last Updated on 06.12.2018 16:11