Genotyping and primer details


Genotyping and primer details for SALK_013557c - At3g06330
Line ID SALK_013557c
Locus At3g06330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1918159 of line SALK_013557c Chr3:1918159
   
Insertion allele amplicon LBb3 - 38ke
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 38ke: 5'-TAGCCATAGGATCTGCTTTTCATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1917775
   
wt amplicon (over InsPos) 19ls - 38ke
wt amplicon start Chr3:1917775
wt amplicon end Chr3:1918605
wt amplicon size 831 bp
Locus specific primer 38ke: 5'-TAGCCATAGGATCTGCTTTTCATT-3'
2nd locus specific primer 19ls: 5'-GATGTCATGTACCTGCACTTGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11