Sequences from confirmation amplicon


Confirmation sequences for line SALK_013557c and hit At3g06330 are shown.
Line ID SALK_013557c
Locus At3g06330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1918159
Primers used for PCR 38ke - LBb3, show primer details
   
Sequence Name 52-K113870-SALK_013557C-38ke
GenBank Accession LN485563 [GenBank]
Sequencing primer gene specific primer, 38ke: 5'-TAGCCATAGGATCTGCTTTTCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K113870-SALK_013557C-38ke
CCTTGGATTGTTTAGACTTATAATTATGATTTCATCTCAAGGATTAATGGCAATGTAAAG
TTGAAGGATTTGTTTTCATGTGAGAAATTGGTTTTAGCATTTTTCGTTTTGTTGTTAGTG
TGTTATGGTGGATATGAGATTGGCCTGAACTTTGTTTGACATGTATTATACTTTATTGGG
TGATCATCAGGTAACAGAAAAATCTACTGAAGATGTGCCGAGCTGCTCTCACTTGGATCT
TTCAATACAAATACCTTCCAGATCACTTCCATTCGGTAACGGCCGTAACCCAAAGGGTTC
ATTGAAGTCCACTCCTTCATTCAAAAGTGGTGTAAACAAATTGACGCTTAGACAACTTAA
TAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGG
GCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGC
TGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 60-K113869-SALK_013557C-R210
GenBank Accession LN485744 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K113869-SALK_013557C-R210
GTCATTTGTTACCCACTTTTGATGAAGGAGTGGACTTCAATGAACCCTTTGGGTTACGGC
CGTTACCGAATGGAAGTGATCTGGAAGGTATTTGTATTGAAAGATCCAAGTGAGAGCAGC
TCGGCACATCTTCAGTAGATTTTTCTGTTACCTGATGATCACCCAATAAAGTATAATACA
TGTCAAACAAAGTTCAGGCCAATCTCATATCCACCATAACACACTAACAACAAAACGAAA
AATGCTAAAACCAATTTCTCACATGAAAACAAATCCTTCAACTTTACATTGCCATTAATC
CTTGAGATGAAATCATAATTATAAGTCTAAAACAATTCCAAGTGGCTTAGTTTGTTCTCT
TAATAAAAGAAAAATGAAAAGCAGACCTTATGGCT

Last Updated on 06.12.2018 16:11