Genotyping and primer details


Genotyping and primer details for SALK_014042 - At1g01550
Line ID SALK_014042
Locus At1g01550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:200695 of line SALK_014042 Chr1:200695
   
Insertion allele amplicon R204 - 48aq
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer 48aq: 5'-TAAGGTACATCAGATTCTGCGATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:201198
   
wt amplicon (over InsPos) 18au - 48aq
wt amplicon start Chr1:200173
wt amplicon end Chr1:201198
wt amplicon size 1026 bp
Locus specific primer 48aq: 5'-TAAGGTACATCAGATTCTGCGATG-3'
2nd locus specific primer 18au: 5'-CTCTTACGCTTGAGTCTTGGATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11