Sequences from confirmation amplicon


Confirmation sequences for line SALK_014042 and hit At1g01550 are shown.
Line ID SALK_014042
Locus At1g01550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:200695
Primers used for PCR 48aq - R204, show primer details
   
Sequence Name 11-K106831-SALK_014042C-R210
GenBank Accession LN484572 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K106831-SALK_014042C-R210
GTCATTTGTTTCCCACAATATATCCTGACTGTCTCATGGATGAAACAAGCTATGGAATCT
CTTTGCGAGACACATAATGGTATCAAGACCCTTATAACCGATCTTGAGCTTCCTGTATCA
GATTGGGAGGATAAATGGGTTGATGTCTATCTTGACATTAGTGTGAAGCTTCTTGATCTC
TGCAATGCCTTTAGCTCTGAGCTTACCCGTCTCAACCAAGGTCATCTCTTACTCCAGTTT
GCTCTGCATAACTTGGAGGCCAACTCTCCTCAGAATCTGTCGAAAGCTCAGTCATCACTA
GACAGTTGGAAGCAACACATTGTCTCCAAGAACCCGAGAATAGAAAATTGCCGTGCAATC
TTGAGCAGCCTTGTTCAGACCTTGAATCTCCCCAAGGTGAAAAACTCGGCCAAAGGGAAA
GTCTTGATGAGAGCTCTATATGGTGTAAAGGTTAAGACCTTATACATCTCTGGTGTTTTT
GCTGCAGCGTTCTCAGGTTCATCGCAGAATCTGATGTACCTT
   
Sequence Name 17-K106832-SALK_014042C-48aq
GenBank Accession LN484733 [GenBank]
Sequencing primer gene specific primer, 48aq: 5'-TAAGGTACATCAGATTCTGCGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K106832-SALK_014042C-48aq
ATGTATAGGTCTTAACCTTTACACCATATAGAGCTCTCATCAAGACTTTCCCTTTGGCCG
AGTTTTTCACCTTGGGGAGATTCAAGGTCTGAACAAGGCTGCTCAAGATTGCACGGCAAT
TTTCTATTCTCGGGTTCTTGGAGACAATGTGTTGCTTCCAACTGTCTAGTGATGACTGAG
CTTTCGACAGATTCTGAGGAGAGTTGGCCTCCAAGTTATGCAGAGCAAACTGGAGTAAGA
GATGACCTTGGTTGAGACGGGTAAGCTCAGAGCTAAAGGCATTGCAGAGATCAAGAAGCT
TCACACTAATGTCAAGATAGACATCAACCCATTTATCCTCCCAATCTGATACAGGAAGCT
CAAGATCGGTTATAAGGGTCTTGATACCATTATGTGTCTCGCAAAGAGATTCCATAGCTT
GTTTCATCCATGAGACAGTCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAAC
TTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAG
ACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAAAGTTGCAGCAACGGNTCC
ACG

Last Updated on 06.12.2018 16:11