Genotyping and primer details


Genotyping and primer details for SALK_014625 - At3g02780
Line ID SALK_014625
Locus At3g02780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:603024 of line SALK_014625 Chr3:603024
   
Insertion allele amplicon LBb3 - 07hi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 07hi: 5'-TCGACTCTGTTTCCTTGTTATCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:602358
   
wt amplicon (over InsPos) 05ng - 07hi
wt amplicon start Chr3:602358
wt amplicon end Chr3:603385
wt amplicon size 1028 bp
Locus specific primer 07hi: 5'-TCGACTCTGTTTCCTTGTTATCCT-3'
2nd locus specific primer 05ng: 5'-TAGAGATTTTCTTGCACATGTTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11