Genotyping and primer details


Genotyping and primer details for SALK_015052 - At5g52860
Line ID SALK_015052
Locus At5g52860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21420893 of line SALK_015052 Chr5:21420893
   
Insertion allele amplicon R212 - 44xl
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer 44xl: 5'-TGACCCAAAACTCTACCGATATACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21421778
   
Insertion allele amplicon R204 - SC12
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer SC12: 5'-GCAGAGACCAATGAGGAAATAGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21420241
   
wt amplicon (over InsPos) 29au - SC12
wt amplicon start Chr5:21420241
wt amplicon end Chr5:21420891
wt amplicon size 651 bp
Locus specific primer SC12: 5'-GCAGAGACCAATGAGGAAATAGAC-3'
2nd locus specific primer 29au: 5'-CAGCCTAGCTTCAAGATTCTGTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11