Genotyping and primer details


Genotyping and primer details for SALK_015166 - At5g57050
Line ID SALK_015166
Locus At5g57050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23089305 of line SALK_015166 Chr5:23089305
   
Insertion allele amplicon LBb3 - 38db
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 38db: 5'-ATAAAAGCTGCCTTACACACCACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23089903
   
wt amplicon (over InsPos) 88dy - 38db
wt amplicon start Chr5:23088948
wt amplicon end Chr5:23089903
wt amplicon size 956 bp
Locus specific primer 38db: 5'-ATAAAAGCTGCCTTACACACCACT-3'
2nd locus specific primer 88dy: 5'-GCGATAGATACCTTAAACCGTCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11