Genotyping and primer details


Genotyping and primer details for SALK_015380 - At5g52430
Line ID SALK_015380
Locus At5g52430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21283210 of line SALK_015380 Chr5:21283210
   
Insertion allele amplicon LBb3 - 14hd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 14hd: 5'-CAGAGCGTTTAAGAGGTATTACGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21282503
   
Insertion allele amplicon LBb3 - 18ii
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 18ii: 5'-TTTTGACTCTTGTGGAGTGGATTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21282343
   
wt amplicon (over InsPos) 03ir - 14hd
wt amplicon start Chr5:21282503
wt amplicon end Chr5:21283394
wt amplicon size 892 bp
Locus specific primer 14hd: 5'-CAGAGCGTTTAAGAGGTATTACGG-3'
2nd locus specific primer 03ir: 5'-TTGAGTTAACAGGTGAAGACGTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11