Sequences from confirmation amplicon


Confirmation sequences for line SALK_015380 and hit At5g52430 are shown.
Line ID SALK_015380
Locus At5g52430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21283210
Primers used for PCR 18ii - LBb3, show primer details
   
Sequence Name 32-K112623-SALK_015380C-18ii
GenBank Accession LN485166 [GenBank]
Sequencing primer gene specific primer, 18ii: 5'-TTTTGACTCTTGTGGAGTGGATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K112623-SALK_015380C-18ii
GCGGCGGAGTATGCCGGGAAAGTTTTGGCGGCGGCTAATTTAGGGATTTGGTCGTTTAAT
CTCTTTGGTGTTTTGATTCCGGTTTATCTACCCAGAGCGTTTAAGAGGTATTACGGTTCT
GATAAGGAGGATTGAAATCCCGGTTTAGTTCCGGTTCCAATAAGGAGAGTCAAAACCCGG
TTTAATTCCTTTGTGGTTCACAGCTCTTGTTGTTTTCCCTTTTTGTTCTGTATATTATTT
CGATGTTTTCTTTTGAACAATTCAGTTTATGTAGTAGTTCACAGTTGTATTAGATTTCTT
ACGATAATAACCGAATCTATGTTTCTGAAAGAACAGAAATGAAAATGTTTTAGTAATGAT
ATAGAATAAGACCATTCACGATCTCAGTTCAAACAATATATTTGTATGAAACAGTAAAAC
AAGAAATAAACAATTTATATCAATGTAACAAGATTAGATGTAGGAGCTGATCCAAGAATA
TTCCAAAGTTTCCAACTACATGAGTACAGAAGTTAACTGCAAATCAAAGCTTTAAGCTTA
TTTATTCAGTGTGTATAAGTTGTTAGAACCCAAATCTCTTACCTTTTTTCACGATCCATG
TTCAAGGTTTGTTACTTATAGGGTTTTATATCTCTGTCTTTGGTAATGAGGAAGAAGAAG
AAGAGATGTCATTTTAATTTGGTTAGCTGACTCCAGATCGTAACCCCGGGAAGAAACTCC
AGCTGTTTCCTGCAACCTTCTCGATATTCTCGTCTTTCGTGTTGTCGAATTTAAATTCTT
TGCTAGATCCAATCGATGAGGAACTCAGCTGTTTTGACGCTNAGACAACTTAATAACACA
TTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCCCAGTGAGACGGGCAACAGC
TGATTGCCCTTCACGCCCGGCCCTGAGAGAGTTGCAGCAAGCGGGTCACGCTGGTTTGCC
CCACAGGCGAAAATCCTGT
   
Sequence Name 08-K112622-SALK_015380C-R210
GenBank Accession LN484492 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K112622-SALK_015380C-R210
TCCTCATCGATTGGATCTAGCAAAGAATTTAAATTCGACAACACGAAAGACGAGAATATC
GAGAAGGTTGCAGGAAACAGCTGGAGTTTCTTCCCGGGGTTACGATCTGGAGTCAGCTAA
CCAAATTAAAATGACATCTCTTCTTCTTCTTCCTCATTACCAAAGACAGAGATATAAAAC
CCTATAAGTAACAAACCTTGAACATGGATCGTGAAAAAAGGTAAGAGATTTGGGTTCTAA
CAACTTATACACACTGAATAAATAAGCTTAAAGCTTTGATTTGCAGTTAACTTCTGTACT
CATGTAGTTGGAAACTTTGGAATATTCTTGGATCAGCTCCTACATCTAATCTTGTTACAT
TGATATAAATTGTTTATTTCTTGTTTTACTGTTTCATACAAATATATTGTTTGAACTGAG
ATCGTGAATGGTCTTATTCTATATCATTACTAAAACATTTTCATTTCTGTTCTTTCAGAA
ACATAGATTCGGTTATTATCGTAAGAAATCTAATACAACTGTGAACTACTACATAAACTG
AATTGTTCAAAAGAAAACATCGAAATAATATACAGAACAAAAAGGGAAAACAACAAGAGC
TGTGAACCACAAAGGAATTAAACCGGGTTTTGACTCTCCTTATTGGAACCGGAACTAAAC
CGGGATTTCAATCCTCCTTATCAGAACCGTAATACCTCTTAAACGCTCTGGGTAGATAAA
CCGGAATCAAAACACCAAAGAGATTAAACGACCAAATCCCTAAATTAGCCGCCGCCAAAA
CTTTCCCGGCATACATCCGCCGCGCAGAAACCGTCCCCGTCACATCCTCCCTCGAGAACT
CACTCATAATCCACTCCACAGGAGTCAA
Primers used for PCR 14hd - LBb3, show primer details
   
Sequence Name 87-K112710-SALK_015380C-14hd
GenBank Accession LN486307 [GenBank]
Sequencing primer gene specific primer, 14hd: 5'-CAGAGCGTTTAAGAGGTATTACGG-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K112710-SALK_015380C-14hd
CGGTTTGTTCGGTTCCATAGGAGAGTCAAACCCGGTTTAATTCCTTTGTGGTTCACAGCT
CTTGTTGTTTTCCCTTTTTGTTCTGTATATTATTTCGATGTTTTCTTTTGAACAATTCAG
TTTATGTAGTAGTTCACAGTTGTATTAGATTTCTTACGATAATAACCGAATCTATGTTTC
TGAAAGAACAGAAATGAAAATGTTTTAGTAATGATATAGAATAAGACCATTCACGATCTC
AGTTCAAACAATATATTTGTATGAAACAGTAAAACAAGAAATAAACAATTTATATCAATG
TAACAAGATTAGATGTAGGAGCTGATCCAAGAATATTCCAAAGTTTCCAACTACATGAGT
ACAGAAGTTAACTGCAAATCAAAGCTTTAAGCTTATTTATTCAGTGTGTATAAGTTGTTA
GAACCCAAATCTCTTACCTTTTTTCACGATCCATGTTCAAGGTTTGTTACTTATAGGGTT
TTATATCTCTGTCTTTGGTAATGAGGAAGAAGAAGAAGAGATGTCATTTTAATTTGGTTA
GCTGACTCCAGATCGTAACCCCGGGAAGAAACTCCAGCTGTTTCCTGCAACCTTCTCGAT
ATTCTCGTCTTTCGTGTTGTCGAATTTAAATTCTTTGCTAGATCCAATCGATGAGGAACT
CAGCTGTTTTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGG
GTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCGGGCCC
TGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTNTGCCCCAGCAGGCGAAAATCCTGTTTG
ATGGTGGT
   
Sequence Name 77-K112709-SALK_015380C-R210
GenBank Accession LN486173 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K112709-SALK_015380C-R210
CTCATCGATTGGATCTAGCAAAGAATTTAAATTCGACAACACGAAAGACGAGAATATCGA
GAAGGTTGCAGGAAACAGCTGGAGTTTCTTCCCGGGGTTACGATCTGGAGTCAGCTAACC
AAATTAAAATGACATCTCTTCTTCTTCTTCCTCATTACCAAAGACAGAGATATAAAACCC
TATAAGTAACAAACCTTGAACATGGATCGTGAAAAAAGGTAAGAGATTTGGGTTCTAACA
ACTTATACACACTGAATAAATAAGCTTAAAGCTTTGATTTGCAGTTAACTTCTGTACTCA
TGTAGTTGGAAACTTTGGAATATTCTTGGATCAGCTCCTACATCTAATCTTGTTACATTG
ATATAAATTGTTTATTTCTTGTTTTACTGTTTCATACAAATATATTGTTTGAACTGAGAT
CGTGAATGGTCTTATTCTATATCATTACTAAAACATTTTCATTTCTGTTCTTTCAGAAAC
ATAGATTCGGTTATTATCGTAAGAAATCTAATACAACTGTGAACTACTACATAAACTGAA
TTGTTCAAAAGAAAACATCGAAATAATATACAGAACAAAAAGGGAAAACAACAAGAGCTG
TGAACCACAAAGGAATTAAACCGGGTTTTGACTCTCCTTATTGGAACCGGAACTAAACCG
GGATTTCAATCCTCCTTATCAGAACCGTAATACCTCTTAAACGCTCT

Last Updated on 06.12.2018 16:11