Genotyping and primer details


Genotyping and primer details for SALK_015405c - At5g13260
Line ID SALK_015405c
Locus At5g13260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4246273 of line SALK_015405c Chr5:4246273
   
Insertion allele amplicon LBb3 - 33fx
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 33fx: 5'-CTCTCTGTGGAAATGGGTCTAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4245521
   
wt amplicon (over InsPos) 08hb - 33fx
wt amplicon start Chr5:4245521
wt amplicon end Chr5:4246373
wt amplicon size 853 bp
Locus specific primer 33fx: 5'-CTCTCTGTGGAAATGGGTCTAAAG-3'
2nd locus specific primer 08hb: 5'-CATAGCTTTGAGAATGGAGAAAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11