Genotyping and primer details


Genotyping and primer details for SALK_015460c - At4g01990
Line ID SALK_015460c
Locus At4g01990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:871394 of line SALK_015460c Chr4:871394
   
Insertion allele amplicon LBb3 - 73by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 73by: 5'-ATTGCAAATGCTTCTGAGGTTTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:871897
   
wt amplicon (over InsPos) 12dk - 73by
wt amplicon start Chr4:871006
wt amplicon end Chr4:871897
wt amplicon size 892 bp
Locus specific primer 73by: 5'-ATTGCAAATGCTTCTGAGGTTTAT-3'
2nd locus specific primer 12dk: 5'-TGTTTGCAAAAGTTCATTGTTCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11