Genotyping and primer details


Genotyping and primer details for SALK_016116 - At1g05680
Line ID SALK_016116
Locus At1g05680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1703227 of line SALK_016116 Chr1:1703227
   
Insertion allele amplicon R204 - AW15
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer AW15: 5'-GTTGTTTCTTGACACACTGTGGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1703527
   
wt amplicon (over InsPos) AW16 - AW15
wt amplicon start Chr1:1702684
wt amplicon end Chr1:1703527
wt amplicon size 844 bp
Locus specific primer AW15: 5'-GTTGTTTCTTGACACACTGTGGAT-3'
2nd locus specific primer AW16: 5'-GGCAAAACAATAACATGAGATCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11