Genotyping and primer details


Genotyping and primer details for SALK_016341 - At2g43500
Line ID SALK_016341
Locus At2g43500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18063592 of line SALK_016341 Chr2:18063592
   
Insertion allele amplicon LBb3 - 23ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 23ux: 5'-AAATGAATTCTCAGTTTCATCGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18063070
   
wt amplicon (over InsPos) 51vl - 23ux
wt amplicon start Chr2:18063070
wt amplicon end Chr2:18064078
wt amplicon size 1009 bp
Locus specific primer 23ux: 5'-AAATGAATTCTCAGTTTCATCGCT-3'
2nd locus specific primer 51vl: 5'-CAGGTCTTAAGCCAACAAAGATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11