Genotyping and primer details


Genotyping and primer details for SALK_016403c - At1g20370
Line ID SALK_016403c
Locus At1g20370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7051788 of line SALK_016403c Chr1:7051788
   
Insertion allele amplicon LBb3 - 47dl
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 47dl: 5'-TGTTGCTATCATGAGGAACTATGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7052419
   
wt amplicon (over InsPos) 15ec - 47dl
wt amplicon start Chr1:7051283
wt amplicon end Chr1:7052419
wt amplicon size 1137 bp
Locus specific primer 47dl: 5'-TGTTGCTATCATGAGGAACTATGC-3'
2nd locus specific primer 15ec: 5'-AGGAGGACTCTTGTCTATGTGGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11