Genotyping and primer details


Genotyping and primer details for SALK_016556c - At1g70440
Line ID SALK_016556c
Locus At1g70440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26550603 of line SALK_016556c Chr1:26550603
   
Insertion allele amplicon LBb3 - 14ty
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 14ty: 5'-GGTAAGATTGTTTAGAACCGGTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26549865
   
wt amplicon (over InsPos) 12uu - 14ty
wt amplicon start Chr1:26549865
wt amplicon end Chr1:26550957
wt amplicon size 1093 bp
Locus specific primer 14ty: 5'-GGTAAGATTGTTTAGAACCGGTCA-3'
2nd locus specific primer 12uu: 5'-CCGACATTGAAATACGTAACCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11