Genotyping and primer details


Genotyping and primer details for SALK_016726c - At2g27080
Line ID SALK_016726c
Locus At2g27080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11566906 of line SALK_016726c Chr2:11566906
   
Insertion allele amplicon LBb3 - 42lm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 42lm: 5'-CACCATCAACATACGTAATCCAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11566480
   
wt amplicon (over InsPos) 20mu - 42lm
wt amplicon start Chr2:11566480
wt amplicon end Chr2:11567247
wt amplicon size 768 bp
Locus specific primer 42lm: 5'-CACCATCAACATACGTAATCCAAG-3'
2nd locus specific primer 20mu: 5'-TCCTACAAATCCAAAAACATCCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11