Genotyping and primer details


Genotyping and primer details for SALK_016942c - At3g52270
Line ID SALK_016942c
Locus At3g52270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19388425 of line SALK_016942c Chr3:19388425
   
Insertion allele amplicon LBb3 - 09rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 09rb: 5'-CTCCAATCTCATTCTTTTCCTGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19388770
   
wt amplicon (over InsPos) 24rz - 09rb
wt amplicon start Chr3:19388067
wt amplicon end Chr3:19388770
wt amplicon size 704 bp
Locus specific primer 09rb: 5'-CTCCAATCTCATTCTTTTCCTGAC-3'
2nd locus specific primer 24rz: 5'-AACAATTATAACCAACGTAACGCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11