Genotyping and primer details


Genotyping and primer details for SALK_017008c - At2g39820
Line ID SALK_017008c
Locus At2g39820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16618873 of line SALK_017008c Chr2:16618873
   
Insertion allele amplicon LBb3 - 15ty
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 15ty: 5'-TGCTTTCTTTTCAAGTTGCTTACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16618503
   
wt amplicon (over InsPos) 31uu - 15ty
wt amplicon start Chr2:16618503
wt amplicon end Chr2:16619166
wt amplicon size 664 bp
Locus specific primer 15ty: 5'-TGCTTTCTTTTCAAGTTGCTTACA-3'
2nd locus specific primer 31uu: 5'-CGATAAACTTCAACACCAAGAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11