Sequences from confirmation amplicon


Confirmation sequences for line SALK_017008c and hit At2g39820 are shown.
Line ID SALK_017008c
Locus At2g39820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16618873
Primers used for PCR 15ty - LBb3, show primer details
   
Sequence Name 25-K117180-SALK_017008C-15ty
GenBank Accession LN485012 [GenBank]
Sequencing primer gene specific primer, 15ty: 5'-TGCTTTCTTTTCAAGTTGCTTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K117180-SALK_017008C-15ty
TCTTCATATGATAACAATAATTGCGAAATTGGTGTCTTTTCCAAACTCACAAATGCTTAT
TGTCTCGTTTCCGCAACATCTGCCTCCGCAAACTTCTTCACGTATATATACATACATACA
CTCTTGAGTCTTCACATTGTCTAATTAAGTTTTCACGTACAAGTTTGGTTTTTCTATGAC
TTGTAATTTTAATTAATTTATTTGTAGTGGTTATGAGTCGAAGTTAAAAGGTGTTATCCC
AATTGTTACTACTTCCATAGGAGGATCAGGGACTATTGGTAGTCTATGTGTCGGAAACAA
AAACGGACACATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATT
GCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT
GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCC
CCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAAAA
   
Sequence Name 04-K117179-SALK_017008C-R210
GenBank Accession LN484381 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K117179-SALK_017008C-R210
CCACAATATATGTGTCCGTTTTTGTTTCCGACACATAGACTACCAATAGTCCCTGATCCT
CCTATGGAAGTAGTAACAATTGGGATAACACCTTTTAACTTCGACTCATAACCACTACAA
ATAAATTAATTAAAATTACAAGTCATAGAAAAACCAAACTTGTACGTGAAAACTTAATTA
GACAATGTGAAGACTCAAGAGTGTATGTATGTATATATACGTGAAGAAGTTTGCGGAGGC
AGATGTTGCGGAAACGAGACAATAAGCATTTGTGAGTTTGGAAAAGACACCAATTTCGCA
ATTATTGTTATCATATTGAAGACCTGCCAAAAACAAGAACAGAAAAAAAAAGAACAATCC
TGTAAGCAACTTGAAAAGAAAGC

Last Updated on 06.12.2018 16:11