Genotyping and primer details


Genotyping and primer details for SALK_017057 - At5g60760
Line ID SALK_017057
Locus At5g60760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24439466 of line SALK_017057 Chr5:24439466
   
Insertion allele amplicon LBb3 - 26jd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 26jd: 5'-TTCACATCGCTTTCATTGAATTAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24438692
   
wt amplicon (over InsPos) 10km - 26jd
wt amplicon start Chr5:24438692
wt amplicon end Chr5:24439993
wt amplicon size 1302 bp
Locus specific primer 26jd: 5'-TTCACATCGCTTTCATTGAATTAC-3'
2nd locus specific primer 10km: 5'-TGTGATGAAAAATACCAGAAGTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11