Genotyping and primer details


Genotyping and primer details for SALK_017090c - At5g60640
Line ID SALK_017090c
Locus At5g60640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24372664 of line SALK_017090c Chr5:24372664
   
Insertion allele amplicon LBb3 - 34dh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 34dh: 5'-GAGAAAGTGTTGACTTCTGGGAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24373247
   
wt amplicon (over InsPos) 89dy - 34dh
wt amplicon start Chr5:24372074
wt amplicon end Chr5:24373247
wt amplicon size 1174 bp
Locus specific primer 34dh: 5'-GAGAAAGTGTTGACTTCTGGGAAC-3'
2nd locus specific primer 89dy: 5'-TTTACATGTCACCGCGAAATAATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11