Genotyping and primer details


Genotyping and primer details for SALK_017111c - At1g71020
Line ID SALK_017111c
Locus At1g71020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26792818 of line SALK_017111c Chr1:26792818
   
Insertion allele amplicon LBb3 - 28mv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 28mv: 5'-CTTTCTCCATTGGCTCAGATAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26792291
   
wt amplicon (over InsPos) 06oj - 28mv
wt amplicon start Chr1:26792291
wt amplicon end Chr1:26793255
wt amplicon size 965 bp
Locus specific primer 28mv: 5'-CTTTCTCCATTGGCTCAGATAAAC-3'
2nd locus specific primer 06oj: 5'-TCGTAACAGCGACTTATCCTTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11