Genotyping and primer details


Genotyping and primer details for SALK_017374c - At3g16620
Line ID SALK_017374c
Locus At3g16620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5659308 of line SALK_017374c Chr3:5659308
   
Insertion allele amplicon LBb3 - 27eq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 27eq: 5'-AAGCTTCTTGAACAGAGGAACAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5658511
   
wt amplicon (over InsPos) 15lt - 27eq
wt amplicon start Chr3:5658511
wt amplicon end Chr3:5659716
wt amplicon size 1206 bp
Locus specific primer 27eq: 5'-AAGCTTCTTGAACAGAGGAACAAG-3'
2nd locus specific primer 15lt: 5'-TTACAAGATAATATTCCAGGGGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11