Sequences from confirmation amplicon


Confirmation sequences for line SALK_017374c and hit At3g16620 are shown.
Line ID SALK_017374c
Locus At3g16620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5659308
Primers used for PCR 27eq - LBb3, show primer details
   
Sequence Name 49-K111283-SALK_017374C-27eq
GenBank Accession LN485478 [GenBank]
Sequencing primer gene specific primer, 27eq: 5'-AAGCTTCTTGAACAGAGGAACAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K111283-SALK_017374C-27eq
TTTACGCGGATGCTTACTTGCCCTGCTCCTCTGTTATTCAGATTAGCACGAGCAATGAGA
TTAGAGGAACGTCCAATGGGTACTTGAGACTGTATATTCCCTCCGATAGCAAGATCGCCA
TGCCAATCCATCACAGAGAGTCCAAGAGTTGATAAAAACCGACCAAGCGGATAATCTTTA
TCTCGAAACTGAGCTTCTAAAGTACCACCATAAGCAACATCTCCACGACTAGTCATTGCT
CCACCTGACATAACCATCCTGAACCGTTTATTAGCAATCAACTTATCTTCGACTTTTAGT
CCCGCGGAAACTGAATCACCTAAGAGCGTAACAGAGAGACCAGCTGCTGCTTTGTTTTTC
CTAAACTTGTTAAATCTTGTTTCACTTCGAATAGTGTACGCTAATTCCTTCCCAGCATTT
TGCATGTCAAAACCTAGGGAAGTTGATCTACCTTCTCCATGTTTAACCGAGCTGGCCAAT
TCTAGCTGCACATGTGCATCCTTCTTGTCCTTTGTCACTTGGCCAGAGAAAGATACTGGT
ATTTTGTCTTTAACAACAAATAGTCGTTCCGCATTCACACCTTCATAACCAATATCATGA
TCCCACCCATGAGTTTCCAGCACTGGCCTAACAAGCCATTGATTGGAGGTATCAAGGTAC
CGGTACCGATGAGTAGGATTGTCAGAGTCAAAAGATGCAGGTAGAGATAAATCTGGCATT
GGAACTGGAACAGATGCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTA
ATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACG
GGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACG
CTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTC
   
Sequence Name 33-K111282-SALK_017374C-R210
GenBank Accession LN485185 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K111282-SALK_017374C-R210
TTTGTTTACCCCAATATATCCTGCATCTGTTCCAGTTCCATGCCAGATTTATCTCTACCT
GCATCTTTTGACTCTGACAATCCTACTCATCGGTACCGGTACCTTGATACCTCCAATCAA
TGGCTTGTTAGGCCAGTGCTGGAAACTCATGGGTGGGATCATGATATTGGTTATGAAGGT
GTGAATGCGGAACGACTATTTGTTGTTAAAGACAAAATACCAGTATCTTTCTCTGGCCAA
GTGACAAAGGACAAGAAGGATGCACATGTGCAGCTAGAATTGGCCAGCTCGGTTAAACAT
GGAGAAGGTAGATCAACTTCCCTAGGTTTTGACATGCAAAATGCTGGGAAGGAATTAGCG
TACACTATTCGAAGTGAAACAAGATTTAACAAGTTTAGGAAAAACAAAGCAGCAGCTGGT
CTCTCTGTTACGCTCTTAGGTGATTCAGTTTCCGCGGGACTAAAAGTCGAAGATAAGTTG
ATTGCTAATAAACGGTTCAGGATGGTTATGTCAGGTGGAGCAATGACTAGTCGTGGAGAT
GTTGCTTATGGTGGTACTTTAGAAGCTCAGTTTCGAGATAAAGATTATCCGCTTGGTCGG
TTTTTATCAACTCTTGGACTCTCTGTGATGGATTGGCATGGCGATCTTGCTATCGGAGGG
AATATACAGTCTCAAGTACCCATTGGACGTTCCTCTAATCTCATTGCTCGTGCTAATCTG
AATAACAGAGGAGCAGGGCAAGTAAGCATCCGCGTAAACAGCTCTGAGCAGCTTCAACTT
GCTGTGGTTGCACTTGTTCCTCTGTTCAAAGAAGCTTAGCACGAGCAATGAGATTAGAGG
AACGTCCAATGGGTACTTGAGACTGTATATTCCCTCCGATAGCAAGATCGCCATGCCAAT
CCATCACAGAGAGTCCAAGAGTTGATAAAAACCGACCAAGCGGGATATCTTTATCTCGAA
ACTGAGCCTCTAAAGTACCACCATAAGCAACATCTCNACGACTAGTCATTGCTCCACCTG
ACATAACCATCCTGAACCGTTTTATAGCAATCAACTTATCTTCGACTTTTAGT

Last Updated on 06.12.2018 16:11