Genotyping and primer details


Genotyping and primer details for SALK_017702c - At3g10350
Line ID SALK_017702c
Locus At3g10350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3209260 of line SALK_017702c Chr3:3209260
   
Insertion allele amplicon LBb3 - 18md
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 18md: 5'-GCATCCAAGAAATCAGGTAAAGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3209732
   
wt amplicon (over InsPos) 04oy - 18md
wt amplicon start Chr3:3208798
wt amplicon end Chr3:3209732
wt amplicon size 935 bp
Locus specific primer 18md: 5'-GCATCCAAGAAATCAGGTAAAGAG-3'
2nd locus specific primer 04oy: 5'-TTTTGCTCAGGTAAACGAAGAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11