Genotyping and primer details


Genotyping and primer details for SALK_017887 - At4g24330
Line ID SALK_017887
Locus At4g24330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12605535 of line SALK_017887 Chr4:12605535
   
Insertion allele amplicon LBb3 - 49dh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 49dh: 5'-AAGACGACGATGTTTCAGAAGATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12606123
   
wt amplicon (over InsPos) 12ej - 49dh
wt amplicon start Chr4:12605146
wt amplicon end Chr4:12606123
wt amplicon size 978 bp
Locus specific primer 49dh: 5'-AAGACGACGATGTTTCAGAAGATT-3'
2nd locus specific primer 12ej: 5'-AATGTTTAGGCTGAAGTCCAAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11