Genotyping and primer details


Genotyping and primer details for SALK_018036 - At1g13160
Line ID SALK_018036
Locus At1g13160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4488468 of line SALK_018036 Chr1:4488468
   
Insertion allele amplicon LBb3 - 29cv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29cv: 5'-GAAGAGGTGAATGACAGTGATGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4487570
   
wt amplicon (over InsPos) 90dy - 29cv
wt amplicon start Chr1:4487570
wt amplicon end Chr1:4488500
wt amplicon size 931 bp
Locus specific primer 29cv: 5'-GAAGAGGTGAATGACAGTGATGAA-3'
2nd locus specific primer 90dy: 5'-CCTCAATATCAAAACTGCAGACAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11