Genotyping and primer details


Genotyping and primer details for SALK_018319 - At3g42670
Line ID SALK_018319
Locus At3g42670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:14757104 of line SALK_018319 Chr3:14757104
   
Insertion allele amplicon LBb3 - 31vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 31vi: 5'-GATGGAGTTGAGAGCCATACTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:14757765
   
wt amplicon (over InsPos) 56vw - 31vi
wt amplicon start Chr3:14756500
wt amplicon end Chr3:14757765
wt amplicon size 1266 bp
Locus specific primer 31vi: 5'-GATGGAGTTGAGAGCCATACTTTC-3'
2nd locus specific primer 56vw: 5'-GACTTTTGATCCTTTCTTTGCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11