Genotyping and primer details


Genotyping and primer details for SALK_018659 - At5g57060
Line ID SALK_018659
Locus At5g57060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23091133 of line SALK_018659 Chr5:23091133
   
Insertion allele amplicon LBb3 - 34fx
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 34fx: 5'-GGGAACATAAATCCTAGGAGGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23091518
   
wt amplicon (over InsPos) 03il - 34fx
wt amplicon start Chr5:23090627
wt amplicon end Chr5:23091518
wt amplicon size 892 bp
Locus specific primer 34fx: 5'-GGGAACATAAATCCTAGGAGGAAG-3'
2nd locus specific primer 03il: 5'-GGAAATTGAAGATCTGGGTCATAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11