Sequences from confirmation amplicon


Confirmation sequences for line SALK_018659 and hit At5g57060 are shown.
Line ID SALK_018659
Locus At5g57060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23091133
Primers used for PCR 34fx - LBb3, show primer details
   
Sequence Name 66-K112557-SALK_018659C-34fx
GenBank Accession LN485898 [GenBank]
Sequencing primer gene specific primer, 34fx: 5'-GGGAACATAAATCCTAGGAGGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K112557-SALK_018659C-34fx
GTCTTAAACAGATAACATGGGATTAATAGGAGAACAAGTACTCACGAAAACCATCCGATT
CCACAACCAAAGCATGGAAGAGGTTTGTCATATGCTCCTGTTTCCATGTCGTCTACATCT
TTTACAAGTGTATATCTTTTTTTCCCACGAGCCATAGCATCTACCAAAAAACAGAGACAG
TGTCTTTTACTTAGTTCACAGTGATAAGAAGGTGTTGACATGAATGCTTCATCTTTCACA
TCTTACGTTTAATATATTCGATGGTGAGAATCAGAATTTCTGAACTTGTATACATGACAA
GACTAGGTGCATAGGCGGTAATAGTTTGTAAAACAAATTGACGCTTAGACAACTTAATAA
CACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCA
ACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGG
TTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCNGGCAAAA
   
Sequence Name 50-K112555-SALK_018659C-R210
GenBank Accession LN485509 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K112555-SALK_018659C-R210
TTTGTTTTCAACTATTACCGCCTATGCACCTAGTCTTGTCATGTATACAAGTTCAGAAAT
TCTGATTCTCACCATCGAATATATTAAACGTAAGATGTGAAAGATGAAGCATTCATGTCA
ACACCTTCTTATCACTGTGAACTAAGTAAAAGACACTGTCTCTGTTTTTTGGTAGATGCT
ATGGCTCGTGGGAAAAAAAGATATACACTTGTAAAAGATGTAGACGACATGGAAACAGGA
GCATATGACAAACCTCTTCCATGCTTTGGTTGTGGAATCGGATGGTTTTCGTGAGTACTT
GTTCTCCTATTAATCCCATGTTATCTGTTTTAAGACCAATCCTGATTTAATCTTCATTTT
GCCTTTAACAGCTTCCTCCTAGGATTTATGTTCC

Last Updated on 06.12.2018 16:11