Genotyping and primer details


Genotyping and primer details for SALK_018700c - At1g79160
Line ID SALK_018700c
Locus At1g79160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29780432 of line SALK_018700c Chr1:29780432
   
Insertion allele amplicon LBb3 - 02jk
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 02jk: 5'-CCAACCATGGACTACGAGTTTATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29779583
   
wt amplicon (over InsPos) 04ll - 02jk
wt amplicon start Chr1:29779583
wt amplicon end Chr1:29780630
wt amplicon size 1048 bp
Locus specific primer 02jk: 5'-CCAACCATGGACTACGAGTTTATT-3'
2nd locus specific primer 04ll: 5'-ACGGTTACTAAGCGGTTACAAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11