Genotyping and primer details


Genotyping and primer details for SALK_018793c - At5g05850
Line ID SALK_018793c
Locus At5g05850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1764679 of line SALK_018793c Chr5:1764679
   
Insertion allele amplicon LBb3 - CE69
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CE69: 5'-CGATCCAAGAATCTACCTCTATGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1763651
   
wt amplicon (over InsPos) 16lt - CE69
wt amplicon start Chr5:1763651
wt amplicon end Chr5:1765100
wt amplicon size 1450 bp
Locus specific primer CE69: 5'-CGATCCAAGAATCTACCTCTATGC-3'
2nd locus specific primer 16lt: 5'-CTCCATTGCATAGTCTCAAAAATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11