Sequences from confirmation amplicon


Confirmation sequences for line SALK_018793c and hit At5g05850 are shown.
Line ID SALK_018793c
Locus At5g05850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1764679
Primers used for PCR CE69 - LBb3, show primer details
   
Sequence Name 30-K108287-SALK_018793C-CE69
GenBank Accession LN485122 [GenBank]
Sequencing primer gene specific primer, CE69: 5'-CGATCCAAGAATCTACCTCTATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K108287-SALK_018793C-CE69
TCAGACAAGTTACATCTTTGACAGCAAACACACTTTTGAATCTATCACAAGTACTGATCC
AAATGTCTAAGAATTCACCCTAAAGAGTCAAAATCCAGAGCAAAGTTTACATCTTTGACA
CATAAACATATCAGAATTCTAACAGATTTGATCAAAAGATTATGAATTTGACCATTAGGG
ACTTAGAAAAACTGACCTGAAGCTGATTGTTATAGAGATTAAGAACAAGGAGACCTTGAA
TCTTTCCAAATGCTTCAGGAAGCAACTTCAATTTCCGACCAGACAAATCAACTCGGTCCA
ATGGATTCTCCGCAGCTTGTTGCAGTATACTCACAACCTCTTCATTGACTTCAACCTCCG
CAGCTTCCTCCTCCGCCACCGCGGCAGCATTCTTCTCGGCAGACTCATAGATCCTCACGA
GCCTCTCCTCTGCTTCTTTCAAAAGCTTCTCATAAGATTCATGAAGCTCGTTAAGCTTAA
GGATCGATTTATACCAAATCTTCTCTTGCTCCACAGCTTCTCTCCTCTTCTGTTCCTTCT
CCGCCACGTCAATATCGTTAGGAGAGAGAGCGATCTCTTCGAAAGATTCCGACAAAGACG
CGTCGATCTCACGGAGCCTAGCACGAGCTCTGTCGACGGTTTCGTGATCGGGTCTTGGTC
CTAGGGTTCGAAGAACAGATCGGGTCTGAGAAACGTCGGCGGTTGCGTTAGTCATGGAGG
CAAGAACATCAGGATGAGCCAAGTGAGGCATCTGAGTCACTATCTCGATAGAGTGGTTTG
ATGATGAAGAAGGATCGGATTTGGATGGTGGTGGCTCGACATCGGAAGAGGAAGAGGATT
TGGCGGTGAATGAAGGGAGACGATCCAGACCTAAGAGAGAACAGGGAAGT
   
Sequence Name 14-K108286-SALK_018793C-R210
GenBank Accession LN484659 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K108286-SALK_018793C-R210
GAACAATAATCGCTTCTTCTTCTTCTTCTTCTTCCTCAATCTCCCAATCTTTTCTTCCAT
CAAGCTCCCAACAATGGCGACGGAGCTTAACCCTAAGAACTTCCCTGTTCTCTCTTATGT
CTTGGATCGTCTCCCTTCATTCACCGCCAAATCCTCTTCCTCTTCCGATGTCGAGCCACC
ACCATCCAAATCCGATCCTTCTTCATCATCAAACCACTCTATCGAGATAGTGACTCAGAT
GCCTCACTTGGCTCATCCTGATGTTCTTGCCTCCATGACTAACGCAACCGCCGACGTTTC
TCAGACCCGATCTGTTCTTCGAACCCTAGGACCAAGACCCGATCACGAAACCGTCGACAG
AGCTCGTGCTAGGCTCCGTGAGATCGACGCGTCTTTGTCGGAATCTTTCGAAGAGATCGC
TCTCTCTCCTAACGATATTGACGTGGCGGAGAAGGAACAGAAGAGGAGAGAAGCTGTGGA
GCAAGAGAAGATTTGGTATAAATCGATCCTTAAGCTTAACGAGCTTCATGAATCTTATGA
GAAGCTTTTGAAAGAAGCAGAGGAGAGGCTCGTGAGGATCTATGAGTCTGCCGAGAAGAA
TGCTGCCGCGGTGGCGGAGGAGGAAGCTGCGGAGGTTGAAGTCAATGAAGAGGTTGTGAG
TATACTGCAACAAGCTGCGGAGAATCCATTGGACCGAGTTGATTTGTCTGGTCGGAAATT
GAAGTTGCTTCCTGAAGCATTTGGAAAGATTCAAGGTCTCCTTGTTCTTAATCTCTATAA
CAATCAGCTTCAGGTCAGTTTTTCTAAGTCCCTAATGGTCAAATTCATAATCTTTTGATC
AAATCTGTTAGAAATCTGATATGTTTATGTGTCAAAGATGTAAACTTTGCTCTGGATTTT
GACTCTTTAGGGTGAATTCTTTGACATTTGGATCAGTACTTGTGATAGATTCAAAAGTGT
GTTTGCTTGTCAAAGATGTAAACTTTGCTTTGGATTTTGAGTCTTTT

Last Updated on 06.12.2018 16:11