Genotyping and primer details


Genotyping and primer details for SALK_019023c - At4g19710
Line ID SALK_019023c
Locus At4g19710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10729266 of line SALK_019023c Chr4:10729266
   
Insertion allele amplicon LBb3 - 29mv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29mv: 5'-TGCAAGGAAGGTACGTTAACTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10728837
   
wt amplicon (over InsPos) 03or - 29mv
wt amplicon start Chr4:10728837
wt amplicon end Chr4:10729602
wt amplicon size 766 bp
Locus specific primer 29mv: 5'-TGCAAGGAAGGTACGTTAACTCTT-3'
2nd locus specific primer 03or: 5'-GCTGATACTTTAAGCGGGAAGATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11