Sequences from confirmation amplicon


Confirmation sequences for line SALK_019023c and hit At4g19710 are shown.
Line ID SALK_019023c
Locus At4g19710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10729266
Primers used for PCR 29mv - LBb3, show primer details
   
Sequence Name 18-K114624-SALK_019023C-29mv
GenBank Accession LN484786 [GenBank]
Sequencing primer gene specific primer, 29mv: 5'-TGCAAGGAAGGTACGTTAACTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K114624-SALK_019023C-29mv
TAGTCTCTCTCATTGAGCCAAACCCTTCTGTTTGTGCAGGTGATTATCCTCGCTCGAGAA
TCTGGACTGAAATTGGACCTCGCTGATCTCCCCATTAGAAGTCTCGTACCAGAACCTCTA
AAAGTATAAGATCTCTCTATATGTATATCTCATTAACAGCAAAACTAACAATGTCTGCAC
TCAATGTCCTTCCTAATTCTACTTTCAGGGATGCACTTCTGTTGAAGAATTCATGGAGAA
ACTCCCACAGTACGATGGAGACCTAGCAAAAGAAAGGCTAGATGCTGAAAACTCTGGGGA
AGTAAGATTATTCACAACAAACGTTTTCCCTTTCGATCAATGTGATCATATTTTAACCAT
ATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTT
AATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCA
CCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAA
AATCCTGTTTGATGGTGGTTC
   
Sequence Name 02-K114623-SALK_019023C-R210
GenBank Accession LN484315 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K114623-SALK_019023C-R210
ACCACAATATATGGTTAAATATGATCACATTGATCGAAAGGGAAAACGTTTGTTGTGAAT
AATCTTACTTCCCCAGAGTTTTCAGCATCTAGCCTTTCTTTTGCTAGGTCTCCATCGTAC
TGTGGGAGTTTCTCCATGAATTCTTCAACAGAAGTGCATCCCTGAAAGTAGAATTAGGAA
GGACATTGAGTGCAGACATTGTTAGTTTTGCTGTTAATGAGATATACATATAGAGAGATC
TTATACTTTTAGAGGTTCTGGTACGAGACTTCTAATGGGGAGATCAGCGAGGTCCAATTT
CAGTCCAGATTCTCGAGCGAGGATAATCACCTGCACAAACAGAAGGGTTTTGGCTCAATG
AGAGAGACTATCATCAGTTTCAGAACTTTACTAGGTTAGTTTGATAGATAGAAGAGTTAA
CGTACTTTCCTTGC

Last Updated on 06.12.2018 16:11