Genotyping and primer details


Genotyping and primer details for SALK_019285c - At3g07190
Line ID SALK_019285c
Locus At3g07190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2286570 of line SALK_019285c Chr3:2286570
   
Insertion allele amplicon LBb3 - 40lm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 40lm: 5'-TTCTCTTCTTTCTCCTTCAGTTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2286208
   
wt amplicon (over InsPos) 16mu - 40lm
wt amplicon start Chr3:2286208
wt amplicon end Chr3:2287084
wt amplicon size 877 bp
Locus specific primer 40lm: 5'-TTCTCTTCTTTCTCCTTCAGTTCG-3'
2nd locus specific primer 16mu: 5'-TCCTTTAAGGGAGCTTGTAATGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11