Sequences from confirmation amplicon


Confirmation sequences for line SALK_019285c and hit At3g07190 are shown.
Line ID SALK_019285c
Locus At3g07190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2286570
Primers used for PCR 40lm - LBb3, show primer details
   
Sequence Name 56-K114145-SALK_019285C-R210
GenBank Accession LN485647 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K114145-SALK_019285C-R210
TTGTTTTAGAACACTTTATAGGTATCTTTCCTACATCAGTATTTAACTGATTTGTGATAT
AGATATCTTTGGCAAGGAGGATCTCTATGTTTGTTTCCACTCCGTTGCTTCCATAGTTGT
GCTTGGTACTGCTTATGTTCACTAAATTTGGGAGGATTTGAAGGAGTGGTGAAATTTGTT
GTGATGATTGGAATCACGTTTTGAGCTCATATGTTCTTGCAGGTGTTGTGCTCTTTTTTG
GCTTCATTATTGACCGGACACATCATTACCTCGAAAAGCTAATCACTTTAAGAAGCAATG
TCGGGTCATCGAAAGGAGAACTAGAAGAACTTAGGAAGGAGAGAATCGAACTGAAGGAGA
AGGAAGAGA
   
Sequence Name 67-K114146-SALK_019285C-40lm
GenBank Accession LN485935 [GenBank]
Sequencing primer gene specific primer, 40lm: 5'-TTCTCTTCTTTCTCCTTCAGTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K114146-SALK_019285C-40lm
CGACATTGCTTCTTAAAGTGATTAGCTTTTCGAGGTAATGATGTGTCCGGTCAATAATGA
AGCCAAAAAAGAGCACAACACCTGCAAGAACATATGAGCTCAAAACGTGATTCCAATCAT
CACAACAAATTTCACCACTCCTTCAAATCCTCCCAAATTTAGTGAACATAAGCAGTACCA
AGCACAACTATGGAAGCAACGGAGTGGAAACAAACATAGAGATCCTCCTTGCCAAAGATA
TCTATATCACAAATCAGTTAAATACTGATGTAGGAAAGATACCTATAAAGTGTTCTTAAA
ACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTG
GTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGA
GAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATG
GTGGTTCCGAA

Last Updated on 06.12.2018 16:11