Genotyping and primer details


Genotyping and primer details for SALK_019407 - At4g16420
Line ID SALK_019407
Locus At4g16420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9264026 of line SALK_019407 Chr4:9264026
   
Insertion allele amplicon LBb3 - 08gq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 08gq: 5'-TCTCAGTTCTCAGACACTTACGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9263063
   
wt amplicon (over InsPos) 13hs - 08gq
wt amplicon start Chr4:9263063
wt amplicon end Chr4:9264176
wt amplicon size 1114 bp
Locus specific primer 08gq: 5'-TCTCAGTTCTCAGACACTTACGGA-3'
2nd locus specific primer 13hs: 5'-GCAGAGTTGAAGACACACAAAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11