Genotyping and primer details


Genotyping and primer details for SALK_019423c - At1g78450
Line ID SALK_019423c
Locus At1g78450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29517859 of line SALK_019423c Chr1:29517859
   
Insertion allele amplicon LBb3 - 35fx
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 35fx: 5'-GATCCGCAACATATCCACTAATTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29517368
   
wt amplicon (over InsPos) 22ir - 35fx
wt amplicon start Chr1:29517368
wt amplicon end Chr1:29518101
wt amplicon size 734 bp
Locus specific primer 35fx: 5'-GATCCGCAACATATCCACTAATTT-3'
2nd locus specific primer 22ir: 5'-CATATTATTTTTGTCGGCCAACTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11