Genotyping and primer details


Genotyping and primer details for SALK_019429c - At2g34380
Line ID SALK_019429c
Locus At2g34380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14514250 of line SALK_019429c Chr2:14514250
   
Insertion allele amplicon LBb3 - 16ty
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16ty: 5'-TCTCTGAAATCCAAACCTTGAACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14513936
   
wt amplicon (over InsPos) 30uu - 16ty
wt amplicon start Chr2:14513936
wt amplicon end Chr2:14514598
wt amplicon size 663 bp
Locus specific primer 16ty: 5'-TCTCTGAAATCCAAACCTTGAACT-3'
2nd locus specific primer 30uu: 5'-AAGGTCAAGCATCTTCAAATCTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11