Genotyping and primer details


Genotyping and primer details for SALK_019501 - At1g22830
Line ID SALK_019501
Locus At1g22830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8078396 of line SALK_019501 Chr1:8078396
   
Insertion allele amplicon LBb3 - 29de
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29de: 5'-GTTCATGGCTCTATTGAGGTTAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8077536
   
wt amplicon (over InsPos) 91dy - 29de
wt amplicon start Chr1:8077536
wt amplicon end Chr1:8078524
wt amplicon size 989 bp
Locus specific primer 29de: 5'-GTTCATGGCTCTATTGAGGTTAGC-3'
2nd locus specific primer 91dy: 5'-CAAGCTGATAAAACTGCAACCATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11