Sequences from confirmation amplicon


Confirmation sequences for line SALK_019501 and hit At1g22830 are shown.
Line ID SALK_019501
Locus At1g22830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8078396
Primers used for PCR 29de - LBb3, show primer details
   
Sequence Name 31-K109819-SALK_019501C-29de
GenBank Accession LN485144 [GenBank]
Sequencing primer gene specific primer, 29de: 5'-GTTCATGGCTCTATTGAGGTTAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K109819-SALK_019501C-29de
ACGCGTTGATTCTATGTATAAAGATTTGGTAAAGTGGATGTTGCTCGGAGATTGTTTGAC
AGGATGTCTGAAAGGGATGCTGTCTCATGGAATGCAATAATCAACTGTTATACTTCAGAA
GAAAAGTTAGGGGAAGCTTTTAAGCTTCTTGATAGAATGTATCTCTCTGGTGTCGAAGCG
AGTATTGTAACTTGGAACACAATAGCTGGAGGTTGTTTGGAGGCAGGAAATTACATAGGG
GCTTTGAATTGTGTTGTTGGGATGAGAAATTGCAATGTAAGAATAGGTTCTGTAGCTATG
ATTAATGGTTTAAAGGCATGCTCACACATTGGAGCGTTAAAGTGGGGGAAAGTATTTCAT
TGCCTTGTGATCCGTAGTTGTAGTTTTTCCCATGATATTGACAATGTTCGGAATTCGTTG
ATCACAATGTACTCAAGATGCAGTGATCTTAGGCACGCGTTTATAGTGTTTCAGCAGGTT
GAAGCAAATAGTTTGAGTACATGGAATTCTATCATTTCCGGTTTTGCATACNACGAGAGA
TCAGAAGAAACCTCTTTCCTGCTCAAGGAGATGTTGCTCTCTGGATTTCACCCAAATCAC
ATTACGCTTGCTAGCATTCTCCCTCTTTTTGCTCGGGTGGGAAACCTGCAAC
   
Sequence Name 15-K109818-SALK_019501C-R210
GenBank Accession LN484692 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K109818-SALK_019501C-R210
GTCATTTGTCCTTTGTCCCTCTTCCTCATCGAATCAATACTCGCTTTGCAGCTATGATTT
CACCGGATTTTGCATACATATCGACAAGGGAGTTCCACAGGATTAAACAGTCCTTGTAGC
TTTGGCGTCTCAGGATGTAGCAGTGAAACTCTTTCCCATGTTGCAGGTTTCCCACCCGAG
CAAAAAGAGGGAGAATGCTAGCAAGCGTAATGTGATTTGGGTGAAATCCAGAGAGCAACA
TCTCCTTGAGCAGGAAAGAGGTTTCTTCTGATCTCTCGTTGTATGCAAAACCGGAAATGA
TAGAATTCCATGTACTCAAACTATTTGCTTCAACCTGCTGAAACACTATAAACGCGTGCC
TAAGATCACTGCATCTTGAGTACATTGTGATCAACGAATTCCGAACATTGTCAATATCAT
GGGAAAAACTACAACTACGGATCACAAGGCAATGAAATACTTTCCCCCACTTTAACGCTC
CAATGTGTGAGCATGCCTTTAAACCATTAATCATAGCTACAGAACCTATTCTTACATTGC
AATTTCTCATCCCAACAACACAATTCAAAGCCCCTATGTAATTTCCTGCCTCCAAACAAC
CTCCAGCTATTGTGTTCCAAGTTACAATACTCGCTTCGACACCAGAGAGATACATTCTAT
CAAGAAGCTTAAAAGCTTCCCCTAACTTTTCTTCTGAAGTATAACAGTTGATTATTGCAT
TCCATGAGACAGCATCCCTTTCAGACATCCTGTCAAACAATCTCCGAGCAACATC

Last Updated on 06.12.2018 16:11