Genotyping and primer details


Genotyping and primer details for SALK_020163c - At1g03790
Line ID SALK_020163c
Locus At1g03790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:955720 of line SALK_020163c Chr1:955720
   
Insertion allele amplicon LBb3 - 47us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 47us: 5'-GTCTCTGTCTCCACCTATGTCTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:955278
   
wt amplicon (over InsPos) 29vx - 47us
wt amplicon start Chr1:955278
wt amplicon end Chr1:956490
wt amplicon size 1213 bp
Locus specific primer 47us: 5'-GTCTCTGTCTCCACCTATGTCTCC-3'
2nd locus specific primer 29vx: 5'-CAAAACATAACACATTTCAGGGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11