Genotyping and primer details


Genotyping and primer details for SALK_020314c - At5g54270
Line ID SALK_020314c
Locus At5g54270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22039170 of line SALK_020314c Chr5:22039170
   
Insertion allele amplicon LBb3 - 30mv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 30mv: 5'-AATGATCTTTGGTATGGACCTGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22038651
   
wt amplicon (over InsPos) 14oj - 30mv
wt amplicon start Chr5:22038651
wt amplicon end Chr5:22039580
wt amplicon size 930 bp
Locus specific primer 30mv: 5'-AATGATCTTTGGTATGGACCTGAC-3'
2nd locus specific primer 14oj: 5'-TGGAATCTTCATCCACTTAGCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11