Genotyping and primer details


Genotyping and primer details for SALK_020772c - At5g43620
Line ID SALK_020772c
Locus At5g43620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17522776 of line SALK_020772c Chr5:17522776
   
Insertion allele amplicon LBb3 - 30vd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 30vd: 5'-ATTGTCAAAGCTTAATCCAACAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17523330
   
wt amplicon (over InsPos) 55vw - 30vd
wt amplicon start Chr5:17522233
wt amplicon end Chr5:17523330
wt amplicon size 1098 bp
Locus specific primer 30vd: 5'-ATTGTCAAAGCTTAATCCAACAGG-3'
2nd locus specific primer 55vw: 5'-GAGTATCGATTCCAACAAGCGTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11