Genotyping and primer details


Genotyping and primer details for SALK_020988c - At1g76300
Line ID SALK_020988c
Locus At1g76300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28625986 of line SALK_020988c Chr1:28625986
   
Insertion allele amplicon LBb3 - 47cy
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 47cy: 5'-AGGCAAACTATTTTGTGTAACGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28625472
   
wt amplicon (over InsPos) 92dy - 47cy
wt amplicon start Chr1:28625472
wt amplicon end Chr1:28626521
wt amplicon size 1050 bp
Locus specific primer 47cy: 5'-AGGCAAACTATTTTGTGTAACGCT-3'
2nd locus specific primer 92dy: 5'-AGCCCAAACGAAAGTTTAGTAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11