Genotyping and primer details


Genotyping and primer details for SALK_021004c - At5g45190
Line ID SALK_021004c
Locus At5g45190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18280239 of line SALK_021004c Chr5:18280239
   
Insertion allele amplicon LBb3 - 48us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 48us: 5'-GTCAAGGTTGGATGGTATTGACTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18280599
   
wt amplicon (over InsPos) 28vx - 48us
wt amplicon start Chr5:18279655
wt amplicon end Chr5:18280599
wt amplicon size 945 bp
Locus specific primer 48us: 5'-GTCAAGGTTGGATGGTATTGACTT-3'
2nd locus specific primer 28vx: 5'-GTCTGCAGAAACAACATGAAGAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11